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Forberedelse av humant myokardvev for langsiktig dyrking
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Forberedelse av humant myokardvev for langsiktig dyrking

Article DOI: 10.3791/63964-v • 10:58 min • June 2nd, 2022
June 2nd, 2022 •
1,2, 1,2, 1,2,3, 4,5, 1,6, 1,2
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, 2German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), 3Division of Experimental Cardiology, Department of Cardiology, Erasmus MC, University Medical Center Rotterdam, 4Institute of Cellular and Molecular Physiology, Friedrich-Alexander-Universität Erlangen-Nürnberg, 5Muscle Research Center Erlangen (MURCE), Friedrich-Alexander-Universität Erlangen-Nürnberg, 6Department of Cardiac Surgery, University Hospital, LMU Munich

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Vi presenterer en protokoll for ex vivo dyrking av humant ventrikkel myokardvev. Det muliggjør detaljert analyse av kontraksjonskraft og kinetikk, samt anvendelse av pre- og etterbelastning for å etterligne det in vivo fysiologiske miljøet nærmere.

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Medisin utgave 184 myokardvev human dyrking ex vivo økt gjennomstrømning sammentrekning
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