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Journal / Developmental Biology / FACS-Isolation and Culture of Fibro-Adipogenic…
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Developmental Biology

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JoVE Journal Developmental Biology
FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle
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FACS-Isolation and Culture of Fibro-Adipogenic Progenitors and Muscle Stem Cells from Unperturbed and Injured Mouse Skeletal Muscle

Article DOI: 10.3791/63983-v • 11:25 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH)

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Summary
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The precise identification of fibro-adipogenic progenitor cells (FAPs) and muscle stem cells (MuSCs) is critical to studying their biological function in physiological and pathological conditions. This protocol provides guidelines for the isolation, purification, and culture of FAPs and MuSCs from adult mouse muscles.

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FACS-isolation Culture Fibro-adipogenic Progenitors Muscle Stem Cells Mouse Skeletal Muscle Fluorescence Activators Of Sorting Pure Populations Biological Role Physiological Conditions Pathological Conditions Forbes Cells Injured Muscle Downstream Techniques Saccharine Transplantation Mudial Mix Analysis Dissection Pad 70% Ethanol Forceps Mouse Belly Skin Opening Horizontally Hind Limb Intermuscular Fat Quadriceps Isolation Fibro-adipogenic Progenitor Cells Tibialis Anterior Extensor Digitorum Longus
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