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Journal / Developmental Biology / FACS-isolamento e coltura di progenitori…
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Developmental Biology

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JoVE Journal Developmental Biology
FACS-isolamento e coltura di progenitori fibro-adipogeni e cellule staminali muscolari da muscolo scheletrico di topo imperturbato e ferito
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FACS-isolamento e coltura di progenitori fibro-adipogeni e cellule staminali muscolari da muscolo scheletrico di topo imperturbato e ferito

Article DOI: 10.3791/63983-v • 11:25 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH)

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L'identificazione precisa delle cellule progenitrici fibro-adipogeniche (FAP) e delle cellule staminali muscolari (MuSC) è fondamentale per studiare la loro funzione biologica in condizioni fisiologiche e patologiche. Questo protocollo fornisce linee guida per l'isolamento, la purificazione e la coltura di FAP e MuSC da muscoli di topo adulti.

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Biologia dello sviluppo Numero 184 progenitori fibro-adipogeni muscolari cellule staminali muscolari (MuSCs) cellule staminali mesenchimali cellule stromali mesenchimali FACS rigenerazione muscolare
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