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JoVE Journal Developmental Biology
Uso de la bisección para reducir el ADN mitocondrial en el ovocito bovino
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Uso de la bisección para reducir el ADN mitocondrial en el ovocito bovino

Article DOI: 10.3791/64060-v • 06:15 min • July 6th, 2022
July 6th, 2022 •
*1, *1, 2, 2, 2, 3, 1
1Department of Animal, Dairy, and Veterinary Sciences, Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research, San Diego Zoo Wildlife Alliance, 3Genus PLC
* These authors contributed equally

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Aquí, presentamos un protocolo para reducir significativamente el número de copias de ADN mitocondrial en un ovocito bovino (P < 0,0001). Este método utiliza la centrifugación y la bisección para reducir sustancialmente las mitocondrias de los ovocitos y puede permitir una mayor probabilidad de desarrollo en los embriones de transferencia nuclear de células somáticas entre especies reconstruidos.

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Biología del Desarrollo Número 185
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