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Developmental Biology

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JoVE Journal Developmental Biology
Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity
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Real Time and Repeated Measurement of Skeletal Muscle Growth in Individual Live Zebrafish Subjected to Altered Electrical Activity

Article DOI: 10.3791/64063-v • 11:41 min • June 16th, 2022
June 16th, 2022 •
*1, *1, 1, 1
1Randall Centre for Cell and Molecular Biophysics, School of Basic and Medical Biosciences, King’s College London
* These authors contributed equally

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Summary
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Optical clarity is a major advantage for cell biological and physiological work in zebrafish. Robust methods for measurement of cell growth in individual animals are described that permit novel insights into how growth of skeletal muscle and neighboring tissues are integrated with whole body growth.

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Real-time Repeated Measurement Skeletal Muscle Growth Zebrafish Altered Electrical Activity Cellular Growth Live Animals In Vivo Spatial Accuracy Temporal Accuracy Muscle Problems Muscle Loss Muscle-wasting Disorders LMA Preparation Heat Block Fish Mounting Larva Transfer Petri Dish Coating
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