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Immunology and Infection

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JoVE Journal Immunology and Infection
A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model
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A Strategy for the Study of IL-9-Producing Lymphoid Cells in the Nippostrongylus brasiliensis Infection Model

Article DOI: 10.3791/64075-v • 08:38 min • March 3rd, 2023
March 3rd, 2023 •
*1, *1, *1, 1, 1, 1
1Departamento de Biología Celular y del Desarrollo, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México
* These authors contributed equally

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Summary
Automatic Translation

IL-9-expressing T and ILC2 cells are induced during N. brasiliensis infection, yet their characterization has been largely overlooked in the infected intestine due to their low frequency and differential kinetics. This protocol describes the isolation of these cells from different target organs and confirmation of their identity via flow cytometry at different infection stages.

Tags

IL-9 Lymphoid Cells Nippostrongylus Brasiliensis Infection Model Pleiotropic Cytokine Antitumor Immunity Allergic Pathologies Helminth Infections CD4+ T Lymphocytes Innate Lymphoid Cells Lung Small Intestine Draining Lymph Nodes Intracellular Staining Hematopoietic Cells Comprehensive Protocol Expression Of IL-9 Lymphoid Tissues Non-lymphoid Tissues Kinetics Of IL-9 Production Mediastinal Lymph Nodes Mesenteric Lymph Nodes Larvae Infection Standardization Of Assays
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