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JoVE Journal
Cancer Research

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使用造血细胞因子接种肿瘤的实验性黑色素瘤免疫治疗模型
 
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使用造血细胞因子接种肿瘤的实验性黑色素瘤免疫治疗模型

Article DOI: 10.3791/64082-v 09:15 min February 24th, 2023
February 24th, 2023

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Summary

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Click here for the English version.

该协议提出了一种癌症免疫治疗模型,使用表达Flt3L的B16-F10黑色素瘤进行基于细胞的肿瘤疫苗接种。该协议演示了程序,包括培养的肿瘤细胞的制备,肿瘤植入,细胞照射,肿瘤生长的测量,肿瘤内免疫细胞的分离和流式细胞术分析。

Tags

癌症研究,第 192 期,Flt3L,DC,B16-F10 黑色素瘤,肿瘤疫苗,先天免疫,肿瘤内免疫细胞
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