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Journal / Cancer Research / Eksperimentel melanom immunterapi model ved hjælp …
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Cancer Research

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JoVE Journal Cancer Research
Eksperimentel melanom immunterapi model ved hjælp af tumorvaccination med et hæmatopoietisk cytokin
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Eksperimentel melanom immunterapi model ved hjælp af tumorvaccination med et hæmatopoietisk cytokin

Article DOI: 10.3791/64082-v • 09:15 min • February 24th, 2023
February 24th, 2023 •
1, 1, 1, 1
1La Jolla Institute for Immunology

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Protokollen præsenterer en kræftimmunterapimodel ved hjælp af cellebaseret tumorvaccination med Flt3L-ekspressive B16-F10 melanom. Denne protokol demonstrerer procedurerne, herunder forberedelse af dyrkede tumorceller, tumorimplantation, cellebestråling, måling af tumorvækst, isolering af intratumorale immunceller og flowcytometrianalyse.

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Kræftforskning udgave 192 Flt3L DC B16-F10 melanom tumorvaccine medfødt immunitet intratumorale immunceller
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