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Imagem de célula única de cérebro inteiro eálise de cérebros de camundongos neonatais intactos usando ressonância magnética, limpeza de tecidos e microscopia de folha de luz…
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Neuroscience
Neuroscience
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Imagem de célula única de cérebro inteiro e análise de cérebros de camundongos neonatais intactos usando ressonância magnética, limpeza de tecidos e microscopia de folha de luz
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Article
DOI:
10.3791/64096-v
•
08:49 min
•
August 1st, 2022
Imagem de célula única de cérebro inteiro e análise de cérebros de camundongos neonatais intactos usando ressonância magnética, limpeza de tecidos e microscopia de folha de luz
August 1st, 2022
•
Felix A. Kyere*1,2, Ian Curtin*1,2, Oleh Krupa1,2, Carolyn M. McCormick1,2, Mustafa Dere3, Sarah Khan3,7, Minjeong Kim7, Tzu-Wen Winnie Wang4,5,6, Qiuhong He4,5,6, Guorong Wu3, Yen-Yu Ian Shih4,5,6, Jason L. Stein1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
Este protocolo descreve métodos para a realização de ressonância magnética, limpeza e imunomarcação de cérebros de camundongos intactos usando iDISCO+, seguido por uma descrição detalhada de imagens usando microscopia de folha de luz e análises a jusante usando NuMorph.