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Kombinera 3D-magnetiskt kraftställdon och multifunktionell fluorescensavbildning för att studera kärnmekanobiologi
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Kombinera 3D-magnetiskt kraftställdon och multifunktionell fluorescensavbildning för att studera kärnmekanobiologi

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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Denna studie presenterar ett nytt protokoll för att direkt applicera mekanisk kraft på cellkärnan genom magnetiska mikrober som levereras in i cytoplasman och för att genomföra samtidig fluorescerande avbildning av levande celler.

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Biologi utgåva 185 Kärnmekanobiologi magnetisk kraft CRISPR/Cas9 mekanotransduktion mjuk aktiv materia ja-associerat protein (YAP) fluorescerande funktionell avbildning digital bildbehandling optogenetik epigenetik
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