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ヒト間葉系幹細胞から小さな細胞外小胞を単離するための簡単なベンチトップろ過法
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Article
DOI:
10.3791/64106-v
•
09:10 min
•
June 23rd, 2022
ヒト間葉系幹細胞から小さな細胞外小胞を単離するための簡単なベンチトップろ過法
June 23rd, 2022
•
Benson Koh1,2, Kian Leong Tan3, Hong Hao Chan4, Qi Hao Daniel Looi2, Moon Nian Lim5, Chee Wun How4, Jia Xian Law1, Jhi Biau Foo6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University
Chapters
Summary
Please note that all translations are automatically generated.
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このプロトコルは、ヒト臍帯由来間葉系幹細胞の小さな細胞外小胞(hUC-MSC-sEV)を、シンプルなラボスケールのベンチトップ設定で分離する方法を示しています。単離されたhUC-MSC-sEVのサイズ分布、タンパク質濃度、sEVマーカー、および形態は、それぞれナノ粒子追跡分析、BCAタンパク質アッセイ、ウェスタンブロット、および透過型電子顕微鏡によって特徴付けられます。