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En enkel stasjonær filtreringsmetode for å isolere små ekstracellulære vesikler fra humane mesenkymale stamceller
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En enkel stasjonær filtreringsmetode for å isolere små ekstracellulære vesikler fra humane mesenkymale stamceller

Article DOI: 10.3791/64106-v • 09:10 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 3, 4, 2, 5, 4, 1, 6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

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Denne protokollen demonstrerer hvordan man isolerer humane navlestrengsavledede mesenkymale stamcellers små ekstracellulære vesikler (hUC-MSC-sEVs) med en enkel benkeplateinnstilling i laboratorieskala. Størrelsesfordelingen, proteinkonsentrasjonen, sEVs markører og morfologi av isolerte hUC-MSC-sEVs er preget av henholdsvis nanopartikkelsporingsanalyse, BCA-proteinanalyse, western blot og transmisjonselektronmikroskop.

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