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检测胎儿组织来源肠的上皮通透性
 
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检测胎儿组织来源肠的上皮通透性

Article DOI: 10.3791/64108-v 07:51 min June 16th, 2022
June 16th, 2022

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Summary

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Click here for the English version.

该协议详细介绍了从胎儿肠道组织建立肠样,一种三维肠道模型。上皮生物标志物的免疫荧光成像用于模型表征。脂多糖(一种细菌内毒素)的顶端暴露,使用显微注射技术以剂量依赖性方式诱导上皮通透性,通过荧光葡聚糖的泄漏来测量。

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医学,第184期,
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