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Test af epitelpermeabilitet i fostervævsafledte enteroider
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Test af epitelpermeabilitet i fostervævsafledte enteroider

Article DOI: 10.3791/64108-v • 07:51 min • June 16th, 2022
June 16th, 2022 •
1, 2, 3, 3, 2
1Morsani College of Medicine, University of South Florida, 2Department of Pediatrics, Morsani College of Medicine, University of South Florida, 3Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida

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Denne protokol beskriver etableringen af enteroider, en tredimensionel tarmmodel, fra føtal tarmvæv. Immunofluorescerende billeddannelse af epitelbiomarkører blev brugt til modelkarakterisering. Apikal eksponering af lipopolysaccharider, et bakterielt endotoksin, ved hjælp af mikroinjektionsteknik induceret epitelpermeabilitet på en dosisafhængig måde målt ved lækage af fluorescerende dextran.

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