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Journal / Genetics / Udvikling af knock-out muskelcellelinjer ved…
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Genetics

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JoVE Journal Genetics
Udvikling af knock-out muskelcellelinjer ved hjælp af Lentivirus-medieret CRISPR / Cas9 genredigering
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
Journal (Genetics)
Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
Journal (Genetics)
Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
 
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Udvikling af knock-out muskelcellelinjer ved hjælp af Lentivirus-medieret CRISPR / Cas9 genredigering

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Protokollen beskriver, hvordan man genererer knock-out myoblaster ved hjælp af CRISPR/Cas9, startende fra design af guide-RNA'er til den cellulære kloning og karakterisering af knock-out klonerne.

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Genetik udgave 184
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