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Journal / Genetics / 렌티바이러스 매개 CRISPR/Cas9 유전자 편집을 이용한 녹아웃 근육 세포주…
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JoVE Journal Genetics
렌티바이러스 매개 CRISPR/Cas9 유전자 편집을 이용한 녹아웃 근육 세포주 개발
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Journal (Genetics)
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
Journal (Genetics)
Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
 
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렌티바이러스 매개 CRISPR/Cas9 유전자 편집을 이용한 녹아웃 근육 세포주 개발

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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이 프로토콜은 가이드 RNA의 설계부터 녹아웃 클론의 세포 클로닝 및 특성화에 이르기까지 CRISPR/Cas9를 사용하여 녹아웃 근세포를 생성하는 방법을 설명합니다.

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유전학 문제 184
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