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Journal / Genetics / Utvikling av knock-out muskelcellelinjer ved…
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Genetics

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JoVE Journal Genetics
Utvikling av knock-out muskelcellelinjer ved hjelp av lentivirusmediert CRISPR / Cas9 genredigering
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Journal (Genetics)
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An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
Journal (Genetics)
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
 
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Utvikling av knock-out muskelcellelinjer ved hjelp av lentivirusmediert CRISPR / Cas9 genredigering

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Protokollen beskriver hvordan du genererer knock-out myoblasts ved hjelp av CRISPR / Cas9, fra utformingen av guide-RNAer til cellulær kloning og karakterisering av knock-out klonene.

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