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Journal / Genetics / Lentivirüs Aracılı CRISPR / Cas9 Gen Düzenleme…
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Genetics

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JoVE Journal Genetics
Lentivirüs Aracılı CRISPR / Cas9 Gen Düzenleme Kullanarak Nakavt Kas Hücre Hatlarının Geliştirilmesi
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Journal (Genetics)
Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
Journal (Genetics)
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
 
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Lentivirüs Aracılı CRISPR / Cas9 Gen Düzenleme Kullanarak Nakavt Kas Hücre Hatlarının Geliştirilmesi

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Protokol, kılavuz-RNA'ların tasarımından hücresel klonlamaya ve nakavt klonlarının karakterizasyonuna kadar CRISPR / Cas9 kullanarak nakavt miyoblastlarının nasıl üretileceğini açıklar.

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