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JoVE Journal Neuroscience
Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Article DOI: 10.3791/64132-v • 11:40 min • June 23rd, 2022
June 23rd, 2022 •
*1, *1, 1
1Department of Chemistry, The George Washington University
* These authors contributed equally

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Summary
Automatic Translation

A neuronal lysosome proximity labeling proteomics protocol is described here to characterize the dynamic lysosomal microenvironment in human induced pluripotent stem cell-derived neurons. Lysosomal membrane proteins and proteins that interact with lysosomes (stably or transiently) can be accurately quantified in this method with excellent intracellular spatial resolution in live human neurons.

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Neuronal Lysosome Interactome Proximity Labeling Proteomics Lysosomal Microenvironment Lysosome Membrane Proteins IPSC-derived Neurons Lysosomal Dysfunction Brain Diseases Molecular Targets Proximity Labeling Procedure Biotin Phenol Hydrogen Peroxide Solution Quench Buffer Lysis Buffer
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