Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Leginon kullanarak Stage Tilt ile Tek Parçacıklı…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Leginon kullanarak Stage Tilt ile Tek Parçacıklı Cryo-EM Veri Toplama
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
Journal (Biochemistry)
Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support
Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
Journal (Biochemistry)
Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
 
Click here for the English version

Leginon kullanarak Stage Tilt ile Tek Parçacıklı Cryo-EM Veri Toplama

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022
July 1st, 2022 •
1, 1, 2, 2,3, 1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Mevcut protokol, kriyo-EM deneylerinde eğimli tek parçacıklı veri toplama için genelleştirilmiş ve uygulanması kolay bir şemayı açıklamaktadır. Böyle bir prosedür, hava-su arayüzüne bağlılık nedeniyle tercihli oryantasyon yanlılığından muzdarip numuneler için yüksek kaliteli bir EM haritası elde etmek için özellikle yararlıdır.

Tags

JoVE'de Bu Ay Sayı 185
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter