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Biochemistry

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JoVE Journal Biochemistry
In vitro Digestão de emulsões em uma única gotícula via troca multifásica de fluidos gastrointestinais simulados
An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
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In vitro Digestão de emulsões em uma única gotícula via troca multifásica de fluidos gastrointestinais simulados

Article DOI: 10.3791/64158-v • 10:20 min • November 18th, 2022
November 18th, 2022 •
1, 2, 3, 1
1Department of Applied Physics, Faculty of Sciences, Campus de Fuentenueva, University of Granada, 2Department of Physical Chemistry, Faculty of Pharmacy, Campus de Cartuja, University of Granada, 3Department of Software Engineering, School of Computer and Telecommunication Engineering, Campus de Aynadamar, University of Granada

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Um balanço de filme de superfície de gota pendente implementado com uma troca multi-subfase, apelidada de OCTOPUS, permite imitar condições digestivas pela troca subfásica sequencial da solução a granel original com fluidos gastrointestinais simulados. A digestão in vitro simulada é monitorada registrando in situ a tensão interfacial da camada interfacial digerida.

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Bioquímica Edição 189
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