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Cancer Research

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JoVE Journal Cancer Research
Industrialiseret, kunstig intelligens-styret lasermikrodissektion til mikroskaleret proteomisk analyse af tumormikromiljøet
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Industrialiseret, kunstig intelligens-styret lasermikrodissektion til mikroskaleret proteomisk analyse af tumormikromiljøet

Article DOI: 10.3791/64171-v • 13:01 min • June 3rd, 2022
June 3rd, 2022 •
*1,2, *1,4, 1,2, 1,2, 1,2, 1, 1,3,4, 1,2,3, 1,3,4
1Women’s Health Integrated Research Center, Gynecologic Cancer Center of Excellence, Department of Obstetrics and Gynecology, Uniformed Services University and Walter Reed National Military Medical Center, 2The Henry M. Jackson Foundation for the Advancement of Military Medicine, 3The John P. Murtha Cancer Center Research Program, Department of Surgery, Uniformed Services University, 4Women’s Health Integrated Research Center, Inova Women’s Service Line, Inova Health System
* These authors contributed equally

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Denne protokol beskriver en arbejdsgang med høj kapacitet til kunstig intelligens-drevet segmentering af patologibekræftede regioner af interesse fra farvede, tynde vævssektionsbilleder til berigelse af histologiopløste cellepopulationer ved hjælp af lasermikrodissektion. Denne strategi omfatter en ny algoritme, der muliggør overførsel af afgrænsninger, der angiver cellepopulationer af interesse direkte til lasermikroskoper.

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Kræftforskning udgave 184 histopatologisk billedanalyse lasermikrodissektion friskfrosset væv formalinfikseret paraffinindlejret væv trykcyklusteknologi tandemmassemærke kvantitativ proteomik
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