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JoVE Journal Neuroscience
Isolierung und direkte neuronale Reprogrammierung von Maus-Astrozyten
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Isolierung und direkte neuronale Reprogrammierung von Maus-Astrozyten

Article DOI: 10.3791/64175-v • 07:25 min • July 7th, 2022
July 7th, 2022 •
1,2,3, 2, 1,2
1Institute of Stem Cell Research, Helmholtz Zentrum München, German Research Center for Environmental Health, 2Department of Physiological Genomics, Biomedical Center Munich, Ludwig-Maximilians University, 3Graduate School of Systemic Neurosciences, BioCenter, Ludwig-Maximilians University

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Hier beschreiben wir ein detailliertes Protokoll zur Erzeugung hochangereicherter Kulturen von Astrozyten, die aus verschiedenen Regionen des Zentralnervensystems postnataler Mäuse stammen, und deren direkte Umwandlung in funktionelle Neuronen durch die erzwungene Expression von Transkriptionsfaktoren.

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Neuroscience Ausgabe 185
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