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JoVE Journal Cancer Research
Verrode fluorescentie-geassocieerde β-galactosidase-sonde voor identificatie en verrijking van senescente tumorcellen door flowcytometrie
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Verrode fluorescentie-geassocieerde β-galactosidase-sonde voor identificatie en verrijking van senescente tumorcellen door flowcytometrie

Article DOI: 10.3791/64176-v • 14:01 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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Een protocol voor fluorescerende, flowcytometrische kwantificering van senescente kankercellen geïnduceerd door chemotherapiegeneesmiddelen in celkweek of in muizentumormodellen wordt gepresenteerd. Optionele procedures omvatten co-immunostaining, monsterfixatie om grote batch- of tijdpuntanalyse te vergemakkelijken en de verrijking van levensvatbare senescente cellen door flowcytometrische sortering.

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Kankeronderzoek Nummer 187 Senescentie kanker chemotherapie flowcytometrie fluorescerende sonde multiplex
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