JoVE Journal
Biochemistry
Biochemistry
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使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性
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Article
DOI:
10.3791/64179-v
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10:08 min
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December 9th, 2022
使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性
December 9th, 2022
•
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
本协议重点介绍了蛋白质印迹技术在研究神经元K-Cl共转运蛋白KCC2的功能和活性方面的应用。该方案描述了通过蛋白质印迹 在 激酶调节位点Thr906/1007处KCC2磷酸化的研究。此外,本文还简要强调了确认KCC2活性的其他方法。