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JoVE Journal
Biochemistry

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使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性
 
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使用蛋白质印迹法研究神经元K-Cl共转运蛋白KCC2的功能和活性

Article DOI: 10.3791/64179-v 10:08 min December 9th, 2022
December 9th, 2022

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Summary

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Click here for the English version.

本协议重点介绍了蛋白质印迹技术在研究神经元K-Cl共转运蛋白KCC2的功能和活性方面的应用。该方案描述了通过蛋白质印迹 激酶调节位点Thr906/1007处KCC2磷酸化的研究。此外,本文还简要强调了确认KCC2活性的其他方法。

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生物化学,第190期,
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