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JoVE Journal
Immunology and Infection
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鉴定甲型流感病毒感染期间切割蛋白质的半胱天冬酶及其基序
 
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鉴定甲型流感病毒感染期间切割蛋白质的半胱天冬酶及其基序

Article DOI: 10.3791/64189-v 08:16 min July 21st, 2022
July 21st, 2022

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甲型流感病毒(IAV)感染激活半胱天冬酶,切割宿主和病毒蛋白,而宿主和病毒蛋白又具有促病毒和抗病毒功能。通过使用抑制剂、RNA干扰、定点诱变以及蛋白质印迹和RT-qPCR技术,鉴定了受感染哺乳动物细胞中切割宿主皮质肌动蛋白和组蛋白脱乙酰酶的半胱天冬酶。

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免疫学与感染,第185期,
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