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JoVE Journal Neuroscience
Mikrofluidik-assisteret selektiv depolarisering af aksonale mitokondrier
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Mikrofluidik-assisteret selektiv depolarisering af aksonale mitokondrier

Article DOI: 10.3791/64196-v • 06:55 min • August 4th, 2022
August 4th, 2022 •
*1,2, *1,2, 2,3,4
1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology
* These authors contributed equally

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Den nuværende protokol beskriver såning og farvning af neuronale mitokondrier i mikrofluidiske kamre. Den flydende trykgradient i disse kamre muliggør selektiv behandling af mitokondrier i axoner for at analysere deres egenskaber som reaktion på farmakologiske udfordringer uden at påvirke cellelegemets rum.

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Neurovidenskab udgave 186 Axon mitokondrier mikrofluidik neurodegeneration
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