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JoVE Journal Neuroscience
Microfluïdica-geassisteerde selectieve depolarisatie van axonale mitochondriën
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Microfluïdica-geassisteerde selectieve depolarisatie van axonale mitochondriën

Article DOI: 10.3791/64196-v • 06:55 min • August 4th, 2022
August 4th, 2022 •
*1,2, *1,2, 2,3,4
1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology
* These authors contributed equally

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Het huidige protocol beschrijft het zaaien en kleuren van neuronale mitochondriën in microfluïdische kamers. De vloeiende drukgradiënt in deze kamers maakt de selectieve behandeling van mitochondriën in axonen mogelijk om hun eigenschappen te analyseren als reactie op farmacologische uitdagingen zonder het cellichaamcompartiment te beïnvloeden.

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Neurowetenschappen Nummer 186 Axon mitochondriën microfluïdica neurodegeneratie
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