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JoVE Journal Neuroscience
Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
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Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Article DOI: 10.3791/64205-v • 10:09 min • September 16th, 2022
September 16th, 2022 •
1,2, 3, 1,2, 1,2, 4, 5, 6, 1,2,6
1Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, 2Division of Multicellular Circuit Dynamics, National Institute for Physiological Sciences, National Institutes of Natural Sciences, 3Department of System Science, Kobe University Graduate School of System Informatics, 4Department of Biology, Graduate School of Sciences, Kobe University, 5Department of Information Science, Kobe University Graduate School of System Informatics, 6Center of Optical Scattering Image Science, Kobe University

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Summary
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We developed a two-photon holographic microscope that can visualize, assess, and manipulate neural activity using high spatiotemporal resolution, with the aim of elucidating the pathogenesis of neuropsychiatric disorders that are associated with abnormal neural activity.

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Neural Activity Two-photon Holographic Microscopy Spatial Temporal Resolution Manipulate Neuroactivity Evaluate Neuroactivity Neuropsychiatric Disorders Pathogens Neurons Organs Head Plate Implantation Dexamethasone Sodium Phosphate Craniotomy Dental Drill Brain Damage Artificial Spinal Fluid ACSF Solution Inflammatory Reactions Pressure Injection System AAV Solution
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