Journal
/
/
En ansigt Endokardial pudepræparat til plan morfogeneseanalyse i museembryoner
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos
DOI:

08:57 min

July 27, 2022

, ,

Chapters

  • 00:04Introduction
  • 00:50Obtention of AVC and/or OFTs
  • 02:31Whole Mount Immunofluorescence of the Mouse Embryonic Heart
  • 04:35Mounting the Mouse Embryonic AVC or OFTs
  • 06:49Results: Analysis of Valve Development in Mouse Embryonic Heart
  • 08:19Conclusion

Summary

Automatic Translation

Klassisk er endokardiet af musens embryonale ventil primordium blevet analyseret ved hjælp af tværgående, koronale eller sagittale sektioner. Vores nye tilgang til en face, todimensionel billeddannelse af endokardiet i valvulogene regioner tillader plan polaritet og celleomlejringsanalyse af endokardiet under ventiludvikling.

Related Videos

Read Article