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Reconstitueren en karakteriseren van Actin-Microtubule Composieten met instelbare motoraangedreven dynamica en mechanica
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Reconstitueren en karakteriseren van Actin-Microtubule Composieten met instelbare motoraangedreven dynamica en mechanica

Article DOI: 10.3791/64228-v • 09:10 min • August 25th, 2022
August 25th, 2022 •
1, 1,2, 1, 1, 1, 3, 3, 1, 1
1Department of Physics and Biophysics, University of San Diego, 2W. M. Keck Science Department, Scripps College, Pitzer College, and Claremont McKenna College, 3Department of Physics, Syracuse University

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Dit artikel presenteert protocollen voor het engineeren en karakteriseren van afstembare driedimensionale composietnetwerken van co-verstrengelde actinefilamenten en microtubuli. Composieten ondergaan actieve herstructurering en ballistische beweging, aangedreven door myosine II- en kinesinemotoren, en worden afgestemd op de relatieve concentraties actine, microtubuli, motoreiwitten en passieve crosslinkers.

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Biologie Uitgave 186 cytoskelet werkzame stof actine microtubuli myosine kinesine composieten biopolymeren in vitro reconstitutie fluorescentie confocale microscopie
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