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Journal / Genetics / Nachweis homologer Rekombinationszwischenprodukte …
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JoVE Journal Genetics
Nachweis homologer Rekombinationszwischenprodukte mittels Proximityligation und quantitativer PCR in Saccharomyces cerevisiae
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Nachweis homologer Rekombinationszwischenprodukte mittels Proximityligation und quantitativer PCR in Saccharomyces cerevisiae

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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Die D-Loop Capture (DLC) und D-Loop Extension (DLE) Assays nutzen das Prinzip der Proximity-Ligation zusammen mit quantitativer PCR, um D-Loop-Bildung, D-Loop-Extension und Produktbildung am Ort eines induzierbaren doppelsträngigen Bruchs in Saccharomyces cerevisiae zu quantifizieren.

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Genetik Ausgabe 187 D-Schleife Genomstabilität Gelenkmolekül Rad51 bruchinduzierte Replikation
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