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Journal / Genetics / Saccharomyces cerevisiae'de Proximity Ligation ve …
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Genetics

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JoVE Journal Genetics
Saccharomyces cerevisiae'de Proximity Ligation ve Kantitatif PCR ile Homolog Rekombinasyon Ara Ürünlerinin Saptanması
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Saccharomyces cerevisiae'de Proximity Ligation ve Kantitatif PCR ile Homolog Rekombinasyon Ara Ürünlerinin Saptanması

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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D-döngü yakalama (DLC) ve D-döngü uzatma (DLE) testleri, Saccharomyces cerevisiae'de indüklenebilir çift sarmallı bir kırılma yerinde D-döngü oluşumunu, D-döngü uzantısını ve ürün oluşumunu ölçmek için kantitatif PCR ile birlikte yakınlık ligasyonu prensibini kullanır.

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Genetik Sayı 187 D-döngüsü genom stabilitesi eklem molekülü Rad51 kırılmaya bağlı replikasyon
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