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Biochemistry

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JoVE Journal Biochemistry
Visualisering af konformationsdynamikken i membranreceptorer ved hjælp af enkeltmolekyle FRET
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Visualisering af konformationsdynamikken i membranreceptorer ved hjælp af enkeltmolekyle FRET

Article DOI: 10.3791/64254-v • 10:59 min • August 17th, 2022
August 17th, 2022 •
1, 1, 1
1Department of Molecular Biosciences, Northwestern University

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Denne undersøgelse præsenterer en detaljeret procedure til udførelse af enkeltmolekyle fluorescensresonansenergioverførsel (smFRET) eksperimenter på G-proteinkoblede receptorer (GPCR'er) ved hjælp af stedsspecifik mærkning via unaturlig aminosyre (UAA) inkorporering. Protokollen giver en trinvis vejledning til smFRET-prøveforberedelse, eksperimenter og dataanalyse.

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Biokemi udgave 186 G-proteinkoblede receptorer (GPCR'er) konformationsdynamik membranreceptorer unaturlige aminosyrer smFRET
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