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Journal / Immunology and Infection / Isolering og kvantificering af Epstein-Barr-virus …
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolering og kvantificering af Epstein-Barr-virus fra P3HR1-cellelinjen
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Isolering og kvantificering af Epstein-Barr-virus fra P3HR1-cellelinjen

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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Denne protokol tillader isolering af Epstein-Barr-viruspartikler fra den humane P3HR1-cellelinje ved inducering af den virale lytiske cyklus med phorbol 12-myristat 13-acetat. DNA ekstraheres efterfølgende fra det virale præparat og udsættes for PCR i realtid for at kvantificere den virale partikelkoncentration.

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Immunologi og infektion udgave 187
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