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Journal / Immunology and Infection / Epstein-Barr Virüsünün P3HR1 Hücre Hattından…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Epstein-Barr Virüsünün P3HR1 Hücre Hattından İzolasyonu ve Nicelleştirilmesi
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Epstein-Barr Virüsünün P3HR1 Hücre Hattından İzolasyonu ve Nicelleştirilmesi

Article DOI: 10.3791/64279-v • 09:14 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1,2
1Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, 2Center for Infectious Diseases Research (CIDR), Faculty of Medicine, American University of Beirut

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Bu protokol, Epstein-Barr virüs parçacıklarının, forbol 12-miristat 13-asetat ile viral litik döngüyü indükledikten sonra insan P3HR1 hücre hattından izole edilmesine izin verir. DNA daha sonra viral preparattan çıkarılır ve viral parçacık konsantrasyonunu ölçmek için gerçek zamanlı PCR'ye tabi tutulur.

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