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Immunology and Infection

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JoVE Journal Immunology and Infection
Trasfezione efficiente di mRNA trascritto in vitro in cellule in coltura utilizzando nanoparticelle peptide-poloxamina
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Trasfezione efficiente di mRNA trascritto in vitro in cellule in coltura utilizzando nanoparticelle peptide-poloxamina

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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Una nanoparticella peptide-poloxamina autoassemblata (PP-sNp) viene sviluppata utilizzando un dispositivo di miscelazione microfluidica per incapsulare e fornire RNA messaggero trascritto in vitro . L'mRNA/PP-sNp descritto potrebbe trasfettare efficacemente le cellule in coltura in vitro.

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Immunologia e infezione Numero 186 Consegna genica RNA messaggero trascritto in vitro sistemi di consegna poloxamina nanoparticelle
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