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Kombination af clearing- og fluorescensmikroskopi til visualisering af ændringer i genekspression og fysiologiske reaktioner på Plasmodiophora brassicae
Measuring Photophysiology of Attached Stage of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer
Journal (Biology)
Measuring Photophysiology of Attached Stage of Colacium sp. by a Cuvette-Type Fast Repetition Rate Fluorometer
Linearization of the Bradford Protein Assay
Journal (Biology)
Linearization of the Bradford Protein Assay
 
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Kombination af clearing- og fluorescensmikroskopi til visualisering af ændringer i genekspression og fysiologiske reaktioner på Plasmodiophora brassicae

Article DOI: 10.3791/64297-v • 06:58 min • August 5th, 2022
August 5th, 2022 •
1, 1, 1, 2, 3, 3, 1
1Integrative Plant Biology Team, Institute of Plant Genetics Polish Academy of Sciences, 2ZMBP-Center for Plant Molecular Biology, University of Tübingen, 3Department of General Botany, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University

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Den nuværende protokol beskriver en optimeret metode til histologisk observation af galler induceret af Plasmodiophora brassicae. Vibrerende sektioner af hypocotyler ryddes før fluorescensbilleddannelse for at studere involvering af transkriptionsfaktorer og phytohormoner under sygdomsprogression. Denne protokol overvinder begrænsninger for harpiksindlejring, hvilket muliggør plantavisualisering af fluorescerende proteiner.

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