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Journal / Developmental Biology / 接合子のCRISPRエレクトロポレーションによるマウスの効率的なゲノム編集…
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JoVE Journal Developmental Biology
接合子のCRISPRエレクトロポレーションによるマウスの効率的なゲノム編集
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接合子のCRISPRエレクトロポレーションによるマウスの効率的なゲノム編集

Article DOI: 10.3791/64302-v • 07:17 min • December 16th, 2022
December 16th, 2022 •
1, 1
1Department of Biomedical Sciences, School of Veterinary Medicine, The University of Pennsylvania

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ここでは、CRISPR RNP接合子のエレクトロポレーション(CRISPR-EZ)と呼ばれる遺伝子改変マウスの効率的な生成を目的とした簡単な手法について説明します。この方法は、編集試薬をエレクトロポレーションにより胚に100%に近い効率で送達する。このプロトコルは、哺乳類胚における点突然変異、小さなゲノム挿入、および欠失に効果的です。

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発生生物学 第190号
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