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JoVE Journal Neuroscience
Human Microglia-like Cells
Generating a Reproducible Model of Mid-Gestational Maternal Immune Activation using Poly(I:C) to Study Susceptibility and Resilience in Offspring
Journal (Neuroscience)
Generating a Reproducible Model of Mid-Gestational Maternal Immune Activation using Poly(I:C) to Study Susceptibility and Resilience in Offspring
The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders
Journal (Neuroscience)
The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders
 
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Human Microglia-like Cells: Differentiation from Induced Pluripotent Stem Cells and In Vitro Live-cell Phagocytosis Assay using Human Synaptosomes

Article DOI: 10.3791/64323-v • 11:19 min • August 18th, 2022
August 18th, 2022 •
1,2, 1
1Department of Neurology, University of Massachusetts Chan Medical School, 2Translation Science Program, Morningside Graduate School of Biomedical Sciences, University of Massachusetts Chan Medical School

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Este protocolo describe el proceso de diferenciación de células madre pluripotentes inducidas por humanos (iPSC) en células similares a la microglía para la experimentación in vitro . También incluimos un procedimiento detallado para generar sinaptosomas humanos a partir de neuronas motoras inferiores derivadas de iPSC que se pueden usar como sustrato para ensayos de fagocitosis in vitro utilizando sistemas de imágenes de células vivas.

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Neurociencia Número 186
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