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Journal / Immunology and Infection / Tyramide Signal Amplification for the…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
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Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Article DOI: 10.3791/64332-v • 09:15 min • October 20th, 2022
October 20th, 2022 •
1,2, 1,2, 1,2, 1,2
1VIB-UGent Center for Inflammation Research, 2Department of Biomedical Molecular Biology, Ghent University

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Summary
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Tyramide signal amplification during immunofluorescent staining enables the sensitive detection of phosphorylated RIPK3 and MLKL during ZBP1-induced necroptosis after HSV-1 infection.

Tags

Tyramide Signal Amplification Immunofluorescent Staining ZBP1-Dependent Phosphorylation RIPK3 MLKL HSV-1 Infection Human Cells Signal Amplification Protocol Necroptosis Induction ZBP1-expressing HT-29 Cells McCoy's 5A Medium Herpes Simplex Virus One Rim Mutant ICP6 Multiplicity Of Infection Necroptosis-inducing Cocktail GSK840 Inhibitor RIPK3 Kinase Activity
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