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Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Article DOI: 10.3791/64382-v • 13:11 min • February 10th, 2023
February 10th, 2023 •
1, 1
1Biochemistry & Molecular Biology, University of Texas Medical Branch

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This article provides a straightforward and clear protocol to label Salmonella secreted effectors using genetic code expansion (GCE) site-specifically and image the subcellular localization of secreted proteins in HeLa cells using direct stochastic optical reconstruction microscopy (dSTORM)

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Super-resolution Imaging Bacterial Secreted Proteins Genetic Code Expansion Labeling Strategies Organic Fluorophores Fluorescent Protein Fusions Zika CO Protein Zika Virus Host Cells LB Medium Chloramphenicol Ampicillin N-minimal Medium TCO Non-canonical Amino Acid Arabinose MgM Media PBS Buffer
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