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Journal / Developmental Biology / CRISPR/Cas9-medieret højeffektiv genmålretning i…
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Developmental Biology

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JoVE Journal Developmental Biology
CRISPR/Cas9-medieret højeffektiv genmålretning i embryonale stamceller til udvikling af genmanipulerede musemodeller
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CRISPR/Cas9-medieret højeffektiv genmålretning i embryonale stamceller til udvikling af genmanipulerede musemodeller

Article DOI: 10.3791/64385-v • 10:57 min • August 24th, 2022
August 24th, 2022 •
*1, *2, 1,2
1Laboratory of Reproductive Systems Biology, Center for Experimental Medicine and Systems Biology, The Institute of Medical Science, The University of Tokyo, 2Research Institute for Microbial Diseases, Osaka University
* These authors contributed equally

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Her præsenterer vi en protokol for udvikling af genetisk modificerede musemodeller ved hjælp af embryonale stamceller, især til stor DNA-knock-in (KI). Denne protokol er indstillet ved hjælp af CRISPR / Cas9 genomredigering, hvilket resulterer i signifikant forbedret KI-effektivitet sammenlignet med den konventionelle homologe rekombinationsmedierede lineariserede DNA-målretningsmetode.

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