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使用碎裂、均质化和连续过滤的组合从牛卵巢中分离小的前窦卵泡
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Biology
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JoVE Journal
Biology
Isolation of Small Preantral Follicles from the Bovine Ovary Using a Combination of Fragmentation, Homogenization, and Serial Filtration
Please note that all translations are automatically generated.
Click here for the English version.
使用碎裂、均质化和连续过滤的组合从牛卵巢中分离小的前窦卵泡
DOI:
10.3791/64423-v
•
09:11 min
•
September 27, 2022
•
Stephanie P. McDonnell
,
Juliana I. Candelaria
,
Amanda J. Morton
,
Anna C. Denicol
1
Department of Animal Science
,
University of California Davis
Chapters
00:05
Introduction
00:55
Ovary Preparation and Chopping Procedure
03:52
Homogenization Procedure
04:56
Filtration Procedure, Searching, and Collecting Follicles
07:08
Results: Follicle Isolation from Bovine Ovaries and Immunofluorescent Localization of Proteins
08:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
推进前窦卵泡生成的研究需要从单个卵巢中分离卵泡的有效方法。这里介绍的是一种简化的机械方案,用于使用组织切碎器和均质器从牛卵巢中分离卵泡。该方法允许从单个卵巢中收集大量活的前窦卵泡。
Tags
Bovine Ovary
Preantral Follicles
Folliculogenesis
Fertility Preservation
Ovarian Biopsy
Fragmentation
Homogenization
Filtration
Isolation Protocol
Cortex
Medulla
Scalpel
PBS
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