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Biology
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AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants
 
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AirID-Based Proximity Labeling for Protein-Protein Interaction in Plants

Article DOI: 10.3791/64428-v 08:36 min September 16th, 2022
September 16th, 2022

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Summary

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Here, we present a step-by-step protocol for performing the proximity labeling (PL) experiment in cucumber (Cucumis sativus L.) using AT4G18020 (APRR2)-AirID protein as a model. The method describes the construction of a vector, the transformation of a construct through agroinfiltration, biotin infiltration, protein extraction, and purification of biotin-labeled proteins through affinity purification technique.

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AirID Proximity Labeling Protein-protein Interaction PL Approaches Modified Ascorbate Peroxidase APEX Escherichia Coli Biotin Ligase BirA BioID TurboID Restrictions Novel Version Plant Systems AT4G18020 (APRR2) Protein Step-by-step Protocol Vector Construction Agroinfiltration Biotin Transformation Protein Extraction Affinity Purification Technique Analyzing PPIs In Plants
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