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Developmental Biology

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JoVE Journal Developmental Biology
Ablación con láser multifotón de centros organizadores de microtúbulos citoplasmáticos en ovocitos de ratón
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Ablación con láser multifotón de centros organizadores de microtúbulos citoplasmáticos en ovocitos de ratón

Article DOI: 10.3791/64439-v • 08:24 min • November 11th, 2022
November 11th, 2022 •
1,2, 3, 1
1Animal Sciences Research Center, University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core, University of Missouri

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Se presenta un protocolo optimizado que permite el agotamiento de los centros organizadores de microtúbulos citoplasmáticos en ovocitos de ratón durante la metafase I utilizando un láser de femtosegundo de infrarrojo cercano.

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Developmental Biology Número 189 Ovocito metafase citoplasmática MTOC meiosis ablación con láser
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