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Quantificação de RNAs circulares usando PCR de gotículas digitais

Article DOI: 10.3791/64464-v • 08:39 min • September 16th, 2022
September 16th, 2022 •
*1,2, *1,2, 1
1Institute of Life Sciences, 2School of Biotechnology, KIIT University
* These authors contributed equally

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Este protocolo descreve o método detalhado de PCR de gotículas digitais (dd-PCR) para quantificação precisa dos níveis de RNA circular (circRNA) em células usando primers divergentes.

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Biologia Edição 187 RNA CIRCULAR RT-PCR primers divergentes quantificação absoluta
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