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JoVE Journal
Chemistry

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基于靶向质谱的低丰度蛋白质蛋白分析中的微量采样
 
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基于靶向质谱的低丰度蛋白质蛋白分析中的微量采样

Article DOI: 10.3791/64473-v 10:21 min January 13th, 2023
January 13th, 2023

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Summary

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Click here for the English version.

提出了一种方案,用于从干燥的血清样品中测定低丰度生物标志物,例如生物标志物前胃泌素释放肽(ProGRP)。抗体包被的磁珠用于蛋白型ProGRP肽的选择性纯化和富集。随后通过液相色谱-串联质谱分析捕获的肽。

Tags

撤稿,第 191 期,DSS,免疫亲和净化,LC-MS/MS,微量采样,蛋白质分析,生物标志物,生物分析,磁珠,胃泌素释放肽
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