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Journal / Bioengineering / CRISPR-Cas Sistemlerine ve Anti-CRISPR…
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Bioengineering

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JoVE Journal Bioengineering
CRISPR-Cas Sistemlerine ve Anti-CRISPR Proteinlerine Dayalı Gen Dijital Devreleri
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CRISPR-Cas Sistemlerine ve Anti-CRISPR Proteinlerine Dayalı Gen Dijital Devreleri

Article DOI: 10.3791/64539-v • 10:46 min • October 18th, 2022
October 18th, 2022 •
*1, *1, 1
1School of Pharmaceutical Science and Technology, Tianjin University
* These authors contributed equally

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CRISPR-Cas sistemleri ve anti-CRISPR proteinleri, Saccharomyces cerevisiae'deki Boole kapılarının şemasına entegre edildi. Yeni küçük mantık devreleri iyi performans gösterdi ve hem dCas9 / dCas12a tabanlı transkripsiyon faktörlerinin hem de anti-CRISPR proteinlerinin özelliklerinin anlaşılmasını derinleştirdi.

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Geri çekme Sayı 188 CRISPR-Cas sistemi dSpCas9 denAsCas12a dLbCas12a tip II-A anti-CRISPR proteinleri tip V-A anti-CRISPR proteinleri gen dijital devreleri Saccharomyces cerevisiae sentetik biyoloji
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