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控制用于3D细胞培养的微孔退火颗粒支架中的颗粒分数
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture
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控制用于3D细胞培养的微孔退火颗粒支架中的颗粒分数

DOI:
10.3791/64554-v

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08:11 min

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October 28, 2022

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1Department of Biomedical Engineering,Duke University, 2Departments of Biomedical Engineering, Neurology, and Dermatology,Duke University

Chapters

  • 00:04Introduction
  • 01:12Drying Hyaluronic Acid (HA)-Norbornene (NB)
  • 02:423D Cell Culture in Microporous Annealed Particle (MAP) Scaffolds
  • 06:03Results: Generating Granular Scaffolds with Controlled Particle Fractions for In Vitro Tissue Engineering
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

最大限度地减少颗粒支架内颗粒组分的变异性有助于可重复的实验。这项工作描述了为 体外 组织工程应用生成具有受控颗粒组分的颗粒支架的方法。

Tags

Particle FractionMicroporous Annealed Particle Scaffolds3D Cell CultureGranular MaterialsMAP ScaffoldsHydrogel ParticlesScaffold PropertiesCell ResponsesWound RepairDrug DeliveryMicrofluidic Microgel GenerationHyaluronic AcidNorborneneMicrogelsLyophilizationPolydimethylsiloxane

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